TB-IGRA Ho'āʻo Diagnostic

ʻO ka wehewehe pōkole:

ʻO ka hoʻāʻo diagnostic TB-IGRA, i kapa ʻia ʻo Interferon Gamma Release Assay, he ELISA no ka ʻike nui ʻana o Interferon Gamma (IFN-γ) e pane i ka in vitro stimulation e Mycobacterium Tuberculosis antigens i loko o ke koko kanaka.Ana ʻo TB-IGRA i ka hoʻoikaika kino o ke kanaka i ka Mycobacterium Tuberculosis.Hoʻohana ʻia ka hoʻāʻo ʻana ma ke ʻano he kōkua i ka hōʻoia ʻana i ka maʻi TB me ka maʻi maʻi Latent Tuberculosis a me ka maʻi Tuberculosis.

ʻO ka pane ʻana o ka immune i hoʻokumu ʻia e ka Mycobacterium tuberculosis ka nui o ka pane kelepona.Ma hope o ka loaʻa ʻana o ka Mycobacterium tuberculosis, hoʻopuka ke kino i nā pūnaʻi T hoʻomanaʻo kikoʻī e holo ana i loko o ke koko peripheral.ʻO ke ana ʻana o IFN-γ kūʻē i ka Mycobacterium tuberculosis ua hōʻike ʻia he ʻano hana maikaʻi no ka ʻike ʻana i ka maʻi TB (nā huna a me ka hana), i kapa ʻia ʻo IFN-γ in vitro release assay (IGRA).ʻO ka ʻokoʻa nui mai ka hoʻāʻo tuberculin (TST) ʻo ia ka IGRA koho i nā antigens kikoʻī i loaʻa wale i ka Mycobacterium tuberculosis akā ʻaʻole i loaʻa i ka BCG a me ka hapa nui o ka mycobacteria non-tuberculous.

E hoʻouna i ka leka uila iā mākou

Huahana Huahana

Kumukānāwai

Hoʻohana ka pahu i ka ʻike hoʻokuʻu interferon-γ no ka Mycobacterium tuberculosis (TB-IGRA) e ana i ka ikaika o ka pane ʻana o ke kino i hoʻopili ʻia e Mycobacterium tuberculosis specific antigen.
Ka Enzyme-linked immunosorbent assay and double antibody sandwich principle.
• Hoʻopili mua ʻia nā microplates me nā antibodies anti IFN-γ.
• Hoʻohui ʻia nā mea hoʻohālikelike e hoʻāʻo ʻia i loko o nā pūnāwai microplate i uhi ʻia i ka antibody, a laila hoʻohui ʻia nā ʻano antibodies horseradish peroxidase (HRP)-conjugated anti IFN-γ i loko o nā pūnāwai.
• IFN-γ, inā loaʻa, e hana i kahi paʻakikī sanwiti me nā antibodies anti IFN-γ a me HRP-conjugated anti IFN-γ antibodies.
• E hoʻomohala ʻia ke kala ma hope o ka hoʻohui ʻana i nā haʻina substrate, a e hoʻololi ʻia ma hope o ka hoʻohui ʻana i nā mea hoʻomaha.Ana 'ia ka absorbance (OD) me ka mea heluhelu ELISA.
• Hoʻopili ʻia ka ʻike IFN-γ i ka hāpana i ka OD i hoʻoholo ʻia.

Nā hiʻohiʻona huahana

ELISA hoʻomaʻamaʻa maikaʻi no ka maʻi TB huna a ikaika

ʻAʻole keakea mai ka lāʻau BCG

Huahana Huahana

Kumukānāwai	ʻO ka hoʻokolohua immunosorbent pili i ka enzyme
ʻAno	Kaʻina Sandwich
Palapala	CE, NMPA
Hoʻohālike	ʻO ke koko holoʻokoʻa
Hōʻike	48T (ʻike i 11 mau laʻana);96T (ʻike i 27 mau laʻana)
Ka mahana mālama	2-8 ℃
Ola ola	12 mahina