Herpes Simplex II IgM ELISA Kit

ʻIke kēia kit i ka Herpes Simplex virus II IgM antibody (HSV2-IgM) i loko o ka serum kanaka a i ʻole plasma samples, polystyrene microwell strips ua uhi mua ʻia me nā antibodies i kuhikuhi ʻia i ke kanaka immunoglobulin M protein (anti-µ chain).Ma hope o ka hoʻohui mua ʻana i ka serum a i ʻole plasma specimens e nānā ʻia, hiki ke hopu ʻia nā antibodies IgM i loko o ka specimen, a e wehe ʻia nā mea ʻē aʻe i hoʻopaʻa ʻole ʻia (me nā antibodies IgG kikoʻī) ma ka holoi ʻana.Ma ka ʻanuʻu ʻelua, ʻo ka HRP (horseradish peroxidase)-conjugated antigens e hoʻopili wale me nā antibodies HSV2 IgM.Ma hope o ka holoi ʻana no ka wehe ʻana i ka HRP-conjugate i hoʻopaʻa ʻole ʻia, hoʻohui ʻia nā hāʻina chromogen i loko o nā pūnāwai.Ma ke alo o (anti- µ)-(HSV2-lgM)-(HSV2 Ag-HRP) immunocomplex, ma hope o ka holoi ʻana i ka pā, ua hoʻohui ʻia ka substrate TMB no ka hoʻomohala kala, a ʻo ka HRP i pili i ka paʻakikī e hoʻoikaika i ka pane ʻana o ka mea hoʻomohala kala. hana i ka mea polū, hoʻohui i 50 µ I o Stop Solution, a huli melemele.Ua hoʻoholo ʻia ka loaʻa ʻana o ka absorbance o ka antibody HSV2-IgM i ka hāpana e kahi mea heluhelu microplate.