ʻO ka maʻi Herpes Simplex I IgM ELISA Kit

ʻIke ʻia kēia kit Herpes Simplex Virus I lgM antibody (HSV1-lgM) i loko o ka serum kanaka a i ʻole nā ​​laʻana plasma, ua uhi mua ʻia nā ʻāpana microwell polystyrene me nā antibodies i kuhikuhi ʻia i nā protein immunoglobulin M kanaka (ke kaulahao anti-µ).Ma hope o ka hoʻohui mua ʻana i ka serum a i ʻole plasma specimens e nānā ʻia, hiki ke hopu ʻia nā antibodies lgM i loko o ka specimen, a e hoʻoneʻe ʻia nā mea ʻē aʻe i hoʻopaʻa ʻole ʻia (me nā antibodies lgG kikoʻī) ma ka holoi ʻana.Ma ka ʻanuʻu ʻelua, ʻo ka HRP (horseradish peroxidase) -conjugated antigens e hoʻopili wale me nā antibodies HSV1 lgM.Ma hope o ka holoi ʻana no ka wehe ʻana i ka HRP-conjugate i hoʻopaʻa ʻole ʻia, hoʻohui ʻia nā hāʻina chromogen i loko o nā pūnāwai.Ma ke alo o (anti- µ) - (HSV1-lgM) - (HSV1 Ag-HRP) immunocomplex, ma hope o ka holoi ʻana i ka pā, ua hoʻohui ʻia ka substrate TMB no ka hoʻomohala kala, a ʻo ka HRP i pili i ka paʻakikī e hoʻoikaika i ka pane ʻana o ka mea hoʻomohala kala. hana i ka mea polū, hoʻohui i 50 µ I o Stop Solution, a huli melemele.Ua hoʻoholo ʻia ka loaʻa ʻana o ka absorbance o ka antibody HSV1-lgM i ka hāpana e kahi mea heluhelu microplate.