Toxoplasma IgG ELISA Kit

ʻIke kēia kit i ka Toxoplasma lgG antibody (TOX-lgG) i loko o ka serum kanaka a i ʻole nā ​​laʻana plasma, ua uhi mua ʻia nā ʻāpana microwell polystyrene me Toxoplasma antigen.Ma hope o ka hoʻohui mua ʻana i ka serum a i ʻole plasma specimens e nānā ʻia, e pili ana i nā antibodies kikoʻī (TOX-lgG-Ab & kekahi lgM-Ab) i loaʻa i nā specimens maʻi e hoʻopaʻa i nā antigens i ka pae paʻa, a e wehe ʻia nā mea ʻē aʻe i hoʻopaʻa ʻole ʻia e ka holoi ʻana.Ma ka ʻanuʻu ʻelua, ʻo ka HRP (horseradish peroxidase) -conjugated anti-human lgG e hoʻopili wale ʻia me nā antibodies TOX lgG.Ma hope o ka holoi ʻana no ka wehe ʻana i ka HRP-conjugate i hoʻopaʻa ʻole ʻia, hoʻohui ʻia nā hopena chromogen i loko o nā pūnāwai.Ma ke alo o (TOX Ag) - (TOX-lgG) - (anti-human lgG-HRP) immunocomplex, ma hope o ka holoi ʻana i ka pā, ua hoʻohui ʻia ka TMB substrate no ka hoʻomohala kala, a ʻo ka HRP i pili i ka paʻakikī e hoʻoikaika i ka hopena o ka mea hoʻomohala kala. no ka hana ʻana i ka mea uliuli, hoʻohui i 50 µ I o Stop Solution, a huli melemele.Ua hoʻoholo ʻia ka loaʻa ʻana o ka absorbance o ka antibody TOX-lgG i ka hāpana e kahi mea heluhelu microplate.