ʻO ke kanaka Cytomegalovirus IgM ELISA Kit

ʻIke kēia kit i ka Human Cytomegalovirus IgM antibody (HCMV-IgM) i loko o ka serum kanaka a i ʻole nā ​​laʻana plasma, ua uhi mua ʻia nā ʻāpana microwell polystyrene me nā antibodies i kuhikuhi ʻia i ka protein immunoglobulin M kanaka (ke kaulahao anti-µ).Ma hope o ka hoʻohui mua ʻana i ka serum a i ʻole plasma specimens e nānā ʻia, hiki ke hopu ʻia nā antibodies IgM i loko o ka specimen, a e wehe ʻia nā mea ʻē aʻe i hoʻopaʻa ʻole ʻia (me nā antibodies IgG kikoʻī) ma ka holoi ʻana.Ma ka ʻanuʻu ʻelua, ʻo ka HRP (horseradish peroxidase)-conjugated antigens e hoʻopili wale me nā antibodies HCMV IgM.Ma hope o ka holoi ʻana no ka wehe ʻana i ka HRP-conjugate i hoʻopaʻa ʻole ʻia, hoʻohui ʻia nā hāʻina chromogen i loko o nā pūnāwai.Ma ke alo o (anti- µ) -(HCMV-lgM) -(HCMV Ag-HRP) immunocomplex, ma hope o ka holoi ʻana i ka pā, ua hoʻohui ʻia ka substrate TMB no ka hoʻomohala ʻana i ka waihoʻoluʻu, a ʻo ka HRP i hoʻopili ʻia i ka paʻakikī e hoʻoikaika i ka hopena o ka mea hoʻomohala kala. hana i ka mea polū, hoʻohui i 50 µI o Stop Solution, a huli melemele.Ua hoʻoholo ʻia ka loaʻa ʻana o ka absorbance o ka antibody HCMV-IgM i ka hāpana e kahi mea heluhelu microplate.