Hepatitis D virus IgM ELISA Kit

Ke ʻike nei kēia pahu i ka maʻi hepatitis D IgM antibody (HDV-IgM) i loko o ka serum kanaka a i ʻole plasma samples, polystyrene microwell strips ua uhi mua ʻia me nā antibodies i kuhikuhi ʻia i nā protein immunoglobulin M kanaka (ke kaulahao anti-μ).Ma hope o ka hoʻohui mua ʻana i ka serum a i ʻole plasma specimens e nānā ʻia, hiki ke hopu ʻia nā antibodies IgM i loko o ka specimen, a e wehe ʻia nā mea ʻē aʻe i hoʻopaʻa ʻole ʻia (me nā antibodies IgG kikoʻī) ma ka holoi ʻana.Ma ka ʻanuʻu ʻelua, ʻo ka HRP (horseradish peroxidase)-conjugated antigens e hana pono wale me HDV IgM antibodies.Ma hope o ka holoi ʻana no ka wehe ʻana i ka HRP-conjugate i hoʻopaʻa ʻole ʻia, hoʻohui ʻia nā hāʻina chromogen i loko o nā pūnāwai.Ma ke alo o (anti-μ) - (HDV-IgM) - (HDV Ag-HRP) immunocomplex, ma hope o ka holoi ʻana i ka pā, ua hoʻohui ʻia ka substrate TMB no ka hoʻomohala ʻana i ke kala, a ʻo ka HRP i pili i ka paʻakikī e hoʻoikaika i ka hopena o ka mea hoʻomohala kala. hana i ka mea uliuli, hoʻohui i 50μl o Stop Solution, a huli melemele.Ua hoʻoholo ʻia ka loaʻa ʻana o ka absorbance o ka antibody HDV-IgM i ka hāpana e kahi mea heluhelu microplate.