Enterovirus 71(EV71) IgM ELISA Kit

ʻO ka wehewehe pōkole:

ʻO Enterovirus 71 IgM (EV71-IgM) ELISA kit he hōʻike immunosorbent pili i ka enzyme no ka ʻike kūpono o nā antibodies IgM-class iā Enterovirus 71 i loko o ka serum kanaka a i ʻole ka plasma.Manaʻo ʻia e hoʻohana ʻia i loko o nā laboratories clinical no ka ʻike a me ka mālama ʻana i nā maʻi e pili ana i ka maʻi me Enterovirus 71.

ʻO ke kanaka enterovirus 71 (EV71), ka lālā hou loa o Enteroviridae, he kumu maʻamau ia o ka maʻi lima, ka wāwae a me ka waha (HFMD) a i kekahi manawa pili pū me nā maʻi koʻikoʻi o ke kikowaena.Piʻi pinepine nā maʻi i Iune a me Iulai.ʻO ka maʻi me EV71 hiki ke ʻano asymptomatic a i ʻole ke kumu o ka maʻi maʻi a me nā ʻūhā.ʻOi aku ka nui o ka pilikia o nā keiki ma lalo o ka ʻelima ma mua o ka poʻe mākua a me nā keiki ʻōpio.

Hoʻouna ʻia ʻo EV71 mai ke kanaka i ke kanaka ma o ka hoʻopili pololei ʻana a ma o ka hoʻohaumia ʻana o nā lima a i ʻole nā ​​​​mea me nā faeces i maʻi ʻia.Hiki i ka huna ʻana o ka ihu a me ka ʻāʻī, kohu a i ʻole ka wai mai nā ʻōpala ke hoʻolaha i ka maʻi.

Hiki ke hoʻokaʻawale ʻia ka EV71 a ʻike ʻia ma ka ʻāʻī a me ka ʻāʻī me ka hoʻohana ʻana i ka hoʻāʻo PCR.Ua ʻike ʻia ʻo Viral RNA i ka wai vesicle ʻili, ke koko a me ka mimi.Hiki ke hoʻokō ʻia ka maʻi ma mua a me ka maʻalahi me ka hoʻohana ʻana i nā serological assays kikoʻī EV71, me nā hoʻokolohua kikoʻī no ka antibody IgM.


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Kumukānāwai

ʻIke kēia kit i ka Enterovirus 71 IgM antibody (EV71-IgM) i loko o ke kanaka serum a i ʻole plasma samples, polystyrene microwell strips ua uhi mua ʻia me nā antibodies i kuhikuhi ʻia i nā protein immunoglobulin M kanaka (anti-μ chain). ke nānā ʻia, hiki ke hopu ʻia nā antibodies IgM i ka specimen, a e wehe ʻia nā mea ʻē aʻe i hoʻopaʻa ʻole ʻia (me nā antibodies IgG kikoʻī) ma ka holoi ʻana.Ma ka ʻanuʻu ʻelua, ʻo ka HRP (horseradish peroxidase)-conjugated antigens e hoʻopili wale me nā antibodies EV71 IgM.Ma hope o ka holoi ʻana no ka wehe ʻana i ka HRP-conjugate i hoʻopaʻa ʻole ʻia, hoʻohui ʻia nā hāʻina chromogen i loko o nā pūnāwai.Ma ke alo o (anti-μ) - (EV71-IgM) - (EV71-Ag-HRP) immunocomplex, ma hope o ka holoi ʻana i ka pā, ua hoʻohui ʻia ka substrate TMB no ka hoʻomohala kala, a ʻo ka HRP pili i ka paʻakikī e hoʻoikaika i ka hopena o ka mea hoʻomohala kala. e hana i ka mea uliuli, hoʻohui i 50μL o Stop Solution, a huli melemele.Ua hoʻoholo ʻia ka loaʻa ʻana o ka absorbance o ka antibody EV71-IgM i ka hāpana e kahi mea heluhelu microplate.

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ʻO kaʻike kiʻekiʻe, ka kiko'ī a me ke kūpaʻa

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Kumukānāwai ʻO ka hoʻokolohua immunosorbent pili i ka enzyme
ʻAno ʻano hopu
Palapala CE
Hoʻohālike ʻO ka serum kanaka / plasma
Hōʻike 48T / 96T
Ka mahana mālama 2-8 ℃
Ola ola 12 mahina

ʻIke Kauoha

Inoa mea kūʻai Puke Hoʻohālike
Enterovirus 71(EV71) IgM ELISA Kit 48T / 96T ʻO ka serum kanaka / plasma

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