Enterovirus 71(EV71) IgM ELISA Kit
Kumukānāwai
ʻIke kēia kit i ka Enterovirus 71 IgM antibody (EV71-IgM) i loko o ke kanaka serum a i ʻole plasma samples, polystyrene microwell strips ua uhi mua ʻia me nā antibodies i kuhikuhi ʻia i nā protein immunoglobulin M kanaka (anti-μ chain). ke nānā ʻia, hiki ke hopu ʻia nā antibodies IgM i ka specimen, a e wehe ʻia nā mea ʻē aʻe i hoʻopaʻa ʻole ʻia (me nā antibodies IgG kikoʻī) ma ka holoi ʻana.Ma ka ʻanuʻu ʻelua, ʻo ka HRP (horseradish peroxidase)-conjugated antigens e hoʻopili wale me nā antibodies EV71 IgM.Ma hope o ka holoi ʻana no ka wehe ʻana i ka HRP-conjugate i hoʻopaʻa ʻole ʻia, hoʻohui ʻia nā hāʻina chromogen i loko o nā pūnāwai.Ma ke alo o (anti-μ) - (EV71-IgM) - (EV71-Ag-HRP) immunocomplex, ma hope o ka holoi ʻana i ka pā, ua hoʻohui ʻia ka substrate TMB no ka hoʻomohala kala, a ʻo ka HRP pili i ka paʻakikī e hoʻoikaika i ka hopena o ka mea hoʻomohala kala. e hana i ka mea uliuli, hoʻohui i 50μL o Stop Solution, a huli melemele.Ua hoʻoholo ʻia ka loaʻa ʻana o ka absorbance o ka antibody EV71-IgM i ka hāpana e kahi mea heluhelu microplate.
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Huahana Huahana
Kumukānāwai | ʻO ka hoʻokolohua immunosorbent pili i ka enzyme |
ʻAno | ʻano hopu |
Palapala | CE |
Hoʻohālike | ʻO ka serum kanaka / plasma |
Hōʻike | 48T / 96T |
Ka mahana mālama | 2-8 ℃ |
Ola ola | 12 mahina |
ʻIke Kauoha
Inoa mea kūʻai | Puke | Hoʻohālike |
Enterovirus 71(EV71) IgM ELISA Kit | 48T / 96T | ʻO ka serum kanaka / plasma |