Enterovirus 71(EV71) IgG ELISA Kit
Kumukānāwai
ʻIke kēia pahu i ka Enterovirus 71 IgG antibody (EV71-IgG) i loko o ka serum kanaka a i ʻole plasma samples, polystyrene microwell strips ua uhi mua ʻia me Enterovirus 71 antigen.Ma hope o ka hoʻohui mua ʻana i ka serum a i ʻole plasma specimens e nānā ʻia, e pili ana i nā antibodies kikoʻī (EV71-IgG-Ab & kekahi IgM-Ab) aia i loko o nā mea maʻi e hoʻopaʻa i nā antigens i ka wā paʻa, a e wehe ʻia nā mea ʻē aʻe i hoʻopaʻa ʻole ʻia e ka holoi ʻana.I ka lua o ka ʻanuʻu, ʻo ka HRP (horseradish peroxidase) -conjugated anti-human IgG e hoʻopili wale ʻia me nā antibodies EV71 IgG.Ma hope o ka holoi ʻana no ka wehe ʻana i ka HRP-conjugate i hoʻopaʻa ʻole ʻia, hoʻohui ʻia nā hāʻina chromogen i loko o nā pūnāwai.Ma ke alo o (EV71 Ag) - (EV71-IgG) - (anti-human IgG-HRP) immunocomplex, ma hope o ka holoi ʻana i ka pā, ua hoʻohui ʻia ka TMB substrate no ka hoʻomohala kala, a ʻo ka HRP i pili i ka paʻakikī e hoʻoikaika i ka hopena o ka mea hoʻomohala kala. e hana i ka mea uliuli, hoʻohui i 50μL o Stop Solution, a huli melemele.Ua hoʻoholo ʻia ka loaʻa ʻana o ka absorbance o ka antibody EV71-IgG i ka hāpana e kahi mea heluhelu microplate.
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Huahana Huahana
Kumukānāwai | ʻO ka hoʻokolohua immunosorbent pili i ka enzyme |
ʻAno | ʻO ke ʻano hana ʻole |
Palapala | CE |
Hoʻohālike | ʻO ka serum kanaka / plasma |
Hōʻike | 48T / 96T |
Ka mahana mālama | 2-8 ℃ |
Ola ola | 12 mahina |
ʻIke Kauoha
Inoa mea kūʻai | Puke | Hoʻohālike |
Enterovirus 71(EV71) IgG ELISA Kit | 48T / 96T | ʻO ka serum kanaka / plasma |