ʻO SARS-COV-2 Huina Hoʻāʻo Ab (Elisa)

Hoʻokumu ʻia ka SARS-CoV-2 Total Ab Test Kit (ELISA) i ka immunoenzymatic assay no ka hoʻoholo ʻana i nā antibodies SARS-CoV-2 i loko o ke kanaka serum, plasma (EDTA, Heparin a i ʻole sodium citrate).Hoʻopili ʻia nā pūnāwai micro plate ma ke ʻano paʻa me ka recombinant SARS-CoV-2 recombinant Receptor Binding Domain protein.I ka hana incubation mua e pili ana i nā antibodies kūikawā (SARS-CoV-2-IgG-Ab & kekahi IgM-Ab) i loaʻa i nā specimens maʻi e hoʻopaʻa i nā antigens i ka pae paʻa.I ka pau ʻana o ka incubation, holoi ʻia nā mea i hoʻopaʻa ʻole ʻia.No ka lua o ka incubation, ua hoʻohui ʻia ʻo SARS-CoV-2 recombinant Receptor Binding Domain protein conjugate (SARS-CoV-2 recombinant Receptor Binding Domain protein peroxidase conjugate) i hoʻopaʻa ʻia i nā antibodies SARS-CoV-2 (me IgG a me IgM) e hopena ai. ka hoʻokumu ʻana o nā immunocomplexes maʻamau.Ma hope o ka lua o ka holoi ʻana no ka wehe ʻana i ka conjugate keu, hoʻohui ʻia ʻo TMB/substrate (step3).Hoʻololi ke kala polū i ka melemele ma hope o ka pau ʻana o ka hopena me ka hopena hoʻomaha.Hoʻoholo ʻia ka absorbance o nā calibrators a me ka specimen ma o ka hoʻohana ʻana i kahi mea heluhelu papa micro ELISA.Loaʻa nā hualoaʻa no nā laʻana maʻi ma ka hoʻohālikelike ʻana me kahi waiwai ʻoki.