ʻO ka hōʻoia ʻana o ka HFRS - Hemorrhagic Fever me ka Renal Syndrome

Kāpae

ʻO ka maʻi maʻi Hantaan (HV) ka maʻi maʻi mua no ka Hemorrhagic Fever me Renal Syndrome (HFRS). ʻO ka HFRS kahi maʻi maʻi maʻi maʻi zoonotic i hōʻike ʻia e ke kuni, ka hemorrhage, a me ka hōʻino ʻana i ka renal. He maʻi koʻikoʻi ka maʻi, piʻi wikiwiki, a me ka nui o ka make nui, e hoʻoweliweli ana i ke olakino lehulehu. ʻO nā rodents (e like me Apodemus agrarius a me Rattus norvegicus) nā waihona waiwai nui a me nā vectors o HV. Loaʻa ka hoʻouna ʻana i ke kanaka ma o ka aerosolized excreta (mimi, feces, saliva), pili pololei, a i ʻole nahu vector. Hiki ke loaʻa ka HFRS i loko o ka makahiki, a ua maʻalahi ka heluna kanaka. Wahi a WHO, 32 mau ʻāina a puni ka honua i hōʻike i ka maʻi HV, me ka nui o ka nui ma Asia Hikina, ʻEulopa, a me nā Balkans.

Nā mea hōʻailona Antibody ma hope o ka maʻi HV

Ma hope o ka maʻi HV, hoʻopuka ka ʻōnaehana pale kino kanaka i nā antibodies kikoʻī, ʻo ia ka HV-IgM a me HV-IgG.

● HV-IgM antibodies: E lawelawe ma ke ʻano he hōʻailona serological o ka maʻi maʻi mua, ʻike pinepine ʻia i loko o nā lā o ka hoʻomaka ʻana o ka hōʻailona, ​​​​a he mea koʻikoʻi ia no ka maʻi o ka maʻi.

● HV-IgG antibodies: Puka ma hope a hiki ke hoʻomau no ke ola, e hōʻike ana i ka maʻi ma mua a i ʻole ke ola kino. ʻO ka hoʻonui ʻana i ʻehā a i ʻole ka nui o ka HV-IgG antibody titer ma waena o nā laʻana serum acute a convalescent no ka maʻi maʻi.

Nā Hana Hana HV maʻamau

ʻO nā ʻano hana hoʻokolohua o kēia manawa no ka ʻike HV e pili ana i ka hoʻokaʻawale virus, PCR, serological ELISA, a me nā immunoassays gula colloidal.

● Hāʻawi ka moʻomeheu Virus a me ka PCR i ka kikoʻī kiʻekiʻe akā he mau manawa, koi ʻenehana, a koi i nā keʻena hoʻokolohua holomua, e kaupalena ana i kā lākou hoʻohana ākea.

● Hāʻawi ka Micro-immunofluorescence (MIF) i ka pololei maikaʻi akā pono ka microscope fluorescence a me ka wehewehe akamai, e kaupalena ana i ka hoʻohana maʻamau.

● ELISA a me ka colloidal gula assays ua apono 'ia i loko o ka clinical hoʻonohonoho ma muli o ko lakou maʻalahi, ka wikiwiki, kiʻekiʻe sensitivity a kiko'ī, a me ka maʻalahi o ka hōʻiliʻili hāpana (serum/plasma).

Hana Huahana

Nā hiʻohiʻona hōʻike HV-IgM/IgG (ELISA) o Beier Bio

● ʻAno Laʻana: Serum, plasma

● Hoʻoheheʻe Laʻana: Hoʻohana nā IgM a me IgG i ka laʻana punawai kumu me kahi dilution 1:11 (100µl sample diluent + 10µl sample), streamlining workflow a hōʻemi i ka hana hana.

● Mākaukau-e-hoʻohana ʻia ka Reagent: Ua mākaukau nā reagent a pau koe wale nō ka holoi holoi (20x concentrated). Kāleka kala no ka ʻike maʻalahi

● Ke Kaʻina Hana Incubation: 30 min / 30 min / 15 min; hiki piha

● Loaʻa hawewe ʻike: 450 nm me 630 nm kuhikuhi

● Nā ʻāpana i uhi ʻia: 96 a i ʻole 48 mau pūnāwai hiki ke nahaha, kēlā me kēia me kahi helu huahana i paʻi ʻia no ka ʻike a me ka maʻalahi.

ʻO nā hiʻohiʻona hōʻike HV-IgM/IgG (Colloidal Gold) o Beier Bio

● ʻAno Laʻana: Serum

● Manawa ʻIke: Nā hopena i loko o 15 mau minuke; ʻaʻole pono nā mea hana hou; kūpono no ka nānā wikiwiki ʻana i ka maʻi outpatient, pilikia, a me ka hoʻopuehu ʻana i nā hoʻonohonoho maʻi

● Ke Kaʻina Hana: E hoʻohui i ka laʻana 10µl i ka laʻana kāleka hōʻike maikaʻi me ka hoʻohana ʻana i kahi dropper; e wehewehe i nā hopena i loko o 15-20 mau minuke

ʻO ka hana maʻi kino o HV-IgM (ELISA), HV-IgG (ELISA), a me HV-IgM/IgG (Colloidal Gold)