Mycoplasma Pneumonia IgM ELISA Kit

ʻIke kēia kit i ka Mycoplasma Pneumoniae IgM (MP-IgM) i loko o ka serum kanaka a i ʻole plasma samples, polystyrene microwell strips ua uhi mua ʻia me nā antibodies i kuhikuhi ʻia i nā protein immunoglobulin M kanaka (anti-μ chain). , hiki ke hopu ʻia nā antibodies IgM i ka specimen, a e wehe ʻia nā mea ʻē aʻe i hoʻopaʻa ʻole ʻia (me nā antibodies IgG kikoʻī) ma ka holoi ʻana.I ka lua o ka ʻanuʻu, ʻo ka HRP (horseradish peroxidase) -conjugated antigens e hoʻopili wale me nā antibodies MP IgM.Ma hope o ka holoi ʻana no ka wehe ʻana i ka HRP-conjugate i hoʻopaʻa ʻole ʻia, hoʻohui ʻia nā hāʻina chromogen i loko o nā pūnāwai.Ma ke alo o (anti-μ) - (MP-IgM) - (MP-Ag-HRP) immunocomplex, ma hope o ka holoi ʻana i ka pā, ua hoʻohui ʻia ka substrate TMB no ka hoʻomohala kala, a ʻo ka HRP i hoʻopili ʻia i ka paʻakikī e hoʻoikaika i ka hopena o ka mea hoʻomohala kala. e hana i ka mea uliuli, hoʻohui i 50μl o Stop Solution, a huli melemele.Ua hoʻoholo ʻia ka loaʻa ʻana o ka absorbance o ka antibody MP-IgM i ka hāpana e kahi mea heluhelu microplate.