Measles Virus (MV) IgG ELISA Kit

ʻIke kēia kit i ka measles virus IgG antibody (MV-IgG) i loko o ka serum kanaka a i ʻole plasma samples, polystyrene microwell strips ua uhi mua ʻia me ka Measles virus antigen.Ma hope o ka hoʻohui mua ʻana i ka serum a i ʻole ka plasma specimens e nānā ʻia, e pili ana i nā antibodies kikoʻī (MV-IgG-Ab & kekahi IgM-Ab) i loaʻa i nā specimens maʻi e hoʻopaʻa i nā antigens i ka pae paʻa, a e wehe ʻia nā mea i hoʻopaʻa ʻole ʻia e ka holoi ʻana.I ka lua o ka ʻanuʻu, ʻo ka HRP (horseradish peroxidase) -conjugated anti-human IgG e hoʻopili wale ʻia me nā antibodies MV IgG.Ma hope o ka holoi ʻana no ka wehe ʻana i ka HRP-conjugate i hoʻopaʻa ʻole ʻia, hoʻohui ʻia nā hāʻina chromogen i loko o nā pūnāwai.Ma ke alo o (MV Ag) - (MV-IgG) - (anti-human IgG-HRP) immunocomplex, ma hope o ka holoi ʻana i ka pā, ua hoʻohui ʻia ka TMB substrate no ka hoʻomohala kala, a ʻo ka HRP i pili i ka paʻakikī e hoʻoikaika i ka hopena o ka mea hoʻomohala kala. e hoʻoulu i ka mea uliuli, hoʻohui i 50μl o Stop Solution, a huli melemele. ʻO ka hele ʻana o ka absorbance o ka MV-IgG antibody i loko o ka hāpana i hoʻoholo ʻia e kahi mea heluhelu microplate.