ʻO ka maʻi hoʻomaʻamaʻa ʻana o ka hanu kanaka IgM ELISA Kit

Ke hoʻohana nei kēia pahu i ke kumu o ka hopu ʻana e ʻike ai i nā antibodies IgM respiratory syncytial virus (HRSV-IgM) i loko o ka serum kanaka a i ʻole plasma samples, ua hoʻopili mua ʻia nā luawai microtiter me ka anti-human-IgM (μ-chain).ʻO ka mea mua, ma hope o ka hoʻohui ʻana i ka laʻana serum o ka specimen e hoʻāʻo ʻia, e hopu ʻia nā antibodies IgM i ka hāpana, a holoi ʻia nā mea ʻē aʻe i hoʻopaʻa ʻole ʻia (me nā antibodies IgG kikoʻī).Ma ka ʻanuʻu ʻelua, hoʻohui ʻia kahi hōʻailona enzyme antigen HRSV a hoʻopaʻa pono ʻia ka HRSV-IgM i ka IgM i hopu ʻia i ka horseradish peroxidase-labeled HRSV recombinant antigen, holoi i nā mea ʻē aʻe i hoʻopaʻa ʻole ʻia, a hoʻomohala i ka waihoʻoluʻu me ka substrate TMB.Ua hoʻoholo ʻia ka hele ʻana a i ʻole ka loaʻa ʻole o ke kanaka hanu syncytial virus IgM antibodies i loko o nā laʻana ma ke ana ʻana i ka absorbance (A-value) me kahi māka enzyme.