Hepatitis E virus IgM ELISA Kit

ʻO ka wehewehe pōkole:

Hoʻohana ʻia ʻo Hepatitis E virus IgM Test Cassette no ka ʻike pono ʻana i nā antibodies Hepatitis E Virus IgM i loko o ka serum kanaka, plasma (EDTA, heparin, sodium citrate) a i ʻole ke koko holoʻokoʻa (EDTA, heparin, sodium citrate).E hoʻohana ʻia ka hoʻāʻo ma ke ʻano he kōkua i ka ʻike ʻana i ka maʻi hepaʻo viral, ka mea i hoʻokumu ʻia e ka Hepatitis E Virus.

ʻO ka maʻi Hepatitis E (HEV) he maʻi maʻi ʻaʻole i uhi ʻia, hoʻokahi stranded RNA virus i hoʻouna mua ʻia ma ke ala fecal-waha, kahe koko a me ka makuahine-fetal paha.ʻO ka maʻi me ka HEV ke kumu o ka maʻi maʻi maʻi maʻi maʻi a me ka maʻi maʻi maʻi maʻi a i ʻole subclinical ate e like me ka Hepatitis A. ʻOiai ʻehā mau genotypes nui o HEV, hoʻokahi wale nō serotype.

ʻO ka maʻi HEV i loko o ke kanaka e hoʻopuka i nā antibodies IgM, IgA a me IgG.HEV-IgM a me HEV-IgA positivity he hōʻailona o ka maʻi HEV nui a i ʻole.Inā maikaʻi ka anti-HEV-IgM a me ka anti-HEV-IgA i hoʻokahi a i ʻole nā mea ʻelua, he hōʻailona ia o ka maʻi HEV hou.Hiki ke hoʻohana ʻia ka loaʻa ʻana o ka maʻi HEV hou, i hui pū ʻia me ka hana o ke ake, no ka hoʻoholo ʻana inā he maʻi maʻi a ʻoi paha ka maʻi.Hiki ke hoʻohana ʻia ka loaʻa ʻana o ka maʻi HEV i loko o ke ake no ka hoʻoholo ʻana inā he maʻi hepatitis E ka maʻi a i ʻole ka hoʻihoʻi ʻana mai ka maʻi hepatitis E.

E hoʻouna i ka leka uila iā mākou

Huahana Huahana

Kumukānāwai

Ke ʻike nei kēia pahu i ka maʻi hepatitis E IgM antibody (HEV-IgM) i loko o ka serum kanaka a i ʻole nā laʻana plasma, ua uhi mua ʻia nā ʻāpana microwell polystyrene me nā antibodies i kuhikuhi ʻia i nā protein immunoglobulin M kanaka (ke kaulahao anti-μ).Ma hope o ka hoʻohui mua ʻana i ka serum a i ʻole plasma specimens e nānā ʻia, hiki ke hopu ʻia nā antibodies IgM i loko o ka specimen, a e wehe ʻia nā mea ʻē aʻe i hoʻopaʻa ʻole ʻia (me nā antibodies IgG kikoʻī) ma ka holoi ʻana.Ma ka lua o ka 'anu'u, 'o ka HRP (horseradish peroxidase)-conjugated antigens e hana pono wale me HEV IgM antibodies.Ma hope o ka holoi ʻana no ka wehe ʻana i ka HRP-conjugate i hoʻopaʻa ʻole ʻia, hoʻohui ʻia nā hāʻina chromogen i loko o nā pūnāwai.Ma ke alo o (anti-μ) - (HEV-IgM) - (HEV Ag-HRP) immunocomplex, ma hope o ka holoi ʻana i ka pā, ua hoʻohui ʻia ka substrate TMB no ka hoʻomohala kala, a ʻo ka HRP i pili i ka paʻakikī e hoʻoikaika i ka hopena o ka mea hoʻomohala kala. hana i ka mea uliuli, hoʻohui i 50μl o Stop Solution, a huli melemele.Ua hoʻoholo ʻia ka loaʻa ʻana o ka komo ʻana o ka antibody HEV-IgM i ka hāpana e kahi mea heluhelu microplate.

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Huahana Huahana

Kumukānāwai	ʻO ka hoʻokolohua immunosorbent pili i ka enzyme
ʻAno	ʻano hopu
Palapala	CE
Hoʻohālike	ʻO ka serum kanaka / plasma
Hōʻike	48T / 96T
Ka mahana mālama	2-8 ℃
Ola ola	12 mahina