ʻAi-Ovarian (AO) Antibody ELISA Kit

ʻIke kēia kit i nā anti-ovarian antibodies (IgG) i loko o nā laʻana serum kanaka e pili ana i ke ʻano indirect, me nā antigens membrane ovarian hoʻomaʻemaʻe i hoʻohana ʻia no ka uhi mua ʻana i nā microwells.

Hoʻomaka ke kaʻina hoʻāʻo me ka hoʻohui ʻana i ka laʻana serum i nā pūnāwai hopena antigen-precoated no ka incubation. Inā loaʻa nā antibodies anti-ovarian i loko o ka laʻana, e hoʻopaʻa pono lākou i nā antigens membrane ovarian i hoʻopaʻa mua ʻia i loko o nā microwells, e hana ana i nā paʻakikī antigen-antibody paʻa. Wehe ʻia nā mea i hoʻopaʻa ʻole ʻia e hōʻoia i ka pololei o ka ʻike.

A laila, hoʻohui ʻia nā antibodies IgG horseradish peroxidase (HRP)-labeled mouse anti-human i nā pūnāwai. Ma hope o ka lua o ka hoʻoulu ʻana, ua hoʻopaʻa ʻia kēia mau antibodies i hoʻopaʻa inoa ʻia i ka enzyme i nā antibodies anti-ovarian i loko o nā paʻakikī antigen-antibody e kū nei, e hana ana i kahi "antigen-antibody-enzyme label" paʻa paʻa.

ʻO ka hope, hoʻohui ʻia ka hopena substrate TMB. Hoʻopili ka HRP i loko o ka paʻakikī i kahi hopena kemika me TMB, e hana ana i kahi hoʻololi kala ʻike ʻia. Ana 'ia ka absorbance (A waiwai) o ka ho'onā hopena me ka microplate heluhelu, a 'o ka 'ike a me ka 'ole o ka anti-ovarian antibodies i loko o ka hāpana e ho'oholo 'ia ma muli o ka hopena absorbance.