Kiko ELISA Anti-Endometrial (EM).

ʻIke kēia kit i nā anti-endometrial antibodies (IgG) i loko o nā laʻana serum kanaka e pili ana i ke ʻano indirect, me nā antigens membrane endometrial hoʻomaʻemaʻe i hoʻohana ʻia no ka uhi ʻana i nā microwells.

Hoʻomaka ke kaʻina hana hoʻāʻo ma ka hoʻohui ʻana i ka laʻana serum i nā pūnāwai hopena antigen-precoated no ka incubation. Inā loaʻa nā antibodies anti-endometrial i loko o ka hāpana, e hoʻopaʻa pono lākou i nā antigens endometrial pre-coated i loko o nā microwells, e hana ana i nā paʻakikī antigen-antibody paʻa. Ma hope o ka wehe ʻana i nā ʻāpana i hoʻopaʻa ʻole ʻia ma o ka holoi ʻana e pale aku i ka hoʻopilikia ʻana, hoʻohui ʻia nā antibodies anti-human IgG iole horseradish peroxidase-labeled mouse.

Ma hope o kekahi hoʻoulu ʻana, hoʻopaʻa ʻia kēia mau antibodies i hoʻopaʻa inoa ʻia i ka enzyme i nā paʻakikī antigen-antibody. Ke hoʻohui ʻia ka substrate TMB, loaʻa kahi hopena kala ma lalo o ka catalysis o ka enzyme. ʻO ka hope, e ana ka mea heluhelu microplate i ka absorbance (A waiwai), i hoʻohana ʻia no ka hoʻoholo ʻana i ka loaʻa ʻana o nā antibodies anti-endometrial (IgG) i ka hāpana.