Anti-Cyclic Citrullinated Peptide (CCP) Antibody ELISA Kit

ʻIke kēia kit i nā anti-cyclic citrullinated peptide antibodies (CCP antibodies) i loko o nā laʻana serum kanaka e pili ana i ke ʻano indirect, me nā antigens cyclic citrullinated peptide antigens i hoʻohana ʻia e like me ka antigen coating.

Hoʻomaka ke kaʻina hoʻāʻo me ka hoʻohui ʻana i ka laʻana serum i nā pūnāwai hopena i uhi mua ʻia me nā antigens hoʻomaʻemaʻe i ʻōlelo ʻia ma mua, a ukali ʻia e kahi manawa incubation. I loko o kēia incubation, inā loaʻa nā antibodies CCP i ka laʻana, e ʻike pono lākou a hoʻopaʻa i nā antigens cyclic citrullinated peptide antigens i uhi ʻia ma nā microwells, e hana ana i nā paʻakikī antigen-antibody paʻa. No ka hōʻoia ʻana i ka pololei o nā ʻanuʻu aʻe, ua hoʻoneʻe ʻia nā ʻāpana i hoʻopaʻa ʻole ʻia i loko o nā pūnāwai hopena ma o kahi kaʻina holoi, e kōkua ana i ka hoʻopau ʻana i nā mea ʻē aʻe o ka serum.

A laila, hoʻohui ʻia nā conjugates enzyme i nā pūnāwai hopena. Ma hope o ka lua o ka incubation, e hoʻopili pono kēia mau enzyme conjugates i nā antigen-antibody complexes i kēia manawa, e hana ana i kahi paʻakikī immune ʻoi aku ka nui o ka antigen, antibody, a me ka conjugate enzyme. Ke hoʻokomo ʻia ka solution substrate TMB i loko o ka ʻōnaehana, hoʻolalelale ka enzyme i loko o ka conjugate i kahi hopena kemika me ka substrate TMB, e hopena i kahi loli ʻike ʻia. ʻO ka ikaika o kēia hopena kala e pili pono ana i ka nui o nā antibodies CCP i loaʻa i ka laʻana serum kumu. ʻO ka hope, hoʻohana ʻia kahi mea heluhelu microplate e ana i ka absorbance (A waiwai) o ka hui ʻana. Ma ke kālailai ʻana i kēia waiwai absorbance, hiki ke hoʻoholo pololei ʻia ke kiʻekiʻe o nā antibodies CCP i loko o ka laʻana, e hāʻawi ana i kumu hilinaʻi no ka hoʻāʻo ʻana a me ka maʻi maʻi.